Jones Clock Tower

Clear Research Group

Bioorganic and Supramolecular Chemistry

Recent Group News

March 2018: Dr. Clear and students Hannah Adamson and Shelby Wakefield present research at the Spring 2018 ACS National Meeting in New Orleans, LA
September 2017: The Clear group is awarded startup funding from the KY NSF EPSCoR
May 2017: The Clear group receives an MSU CISR grant
February 2017: The Clear group receives the 2017 Marshall and Annette Gordon chemistry research grant

Research in the Clear Group

Research in the Clear Lab integrates themes from organic, physical, and analytical chemistry to address questions regarding recognition of biologically important anions. The emphasis of projects currently underway in the group is on the development of synthetic receptors for solution and membrane recognition of inositol phosphates and phosphatidylinositide lipids, a class of molecues that are important for cell communication. The results of this research will contribute to the scientific knowledge that will be both directly and indirectly beneficial to biological or biochemical separations, understanding of signaling pathways and lipid dynamics, molecular imaging, and therapeutic applications. Importantly, students participating in research will be exposed to a range of techniques, from traditional organic synthesis to binding studies requiring repetitive measurement and extensive data analysis. As a result, this research experience will help train students for graduate and professional studies as well as careers in industry.

1. Solution Anion Recognition

2. Membrane Anion Recognition

3. Assays for Lipid Receptor Affinity & Selectivity

1. Solution recognition of the inositol phosphates using zinc(II) coordination complexes.
The underlying hypothesis is that synthetic receptors with multiple anion recognition units and appropriate scaffolds will exhibit high affinity and selectivity for particular isomers of the inositol phosphates. To test this, a series of bivalent synthetic receptors containing zinc(II)-cyclen and zinc(II)-dipcolylamine anion recognition units presented on a chiral scaffold will be synthesized and the binding toward a series of inositol triphosphate isoforms will be evaluated using isothermal titration calorimetry (ITC). ITC is a technique which measures the heat generated upon association of the two molecules and gives detailed information on the thermodymaics of the binding event.

2. Membrane recognition of the phosphitidylinositol phosphate lipids using zinc(II)-cyclen complexes
The membrane is known to play an important role in molecular recognition mechanisms. The project will use methods like ITC to compare the binding thermodynamics of a bivalent zinc(II)-cyclen receptor with phosphatidylinositol(4,5)bisphosphate (PI(4,5)P2) to association of the same receptor with a soluble analog of PI(4,5)P2, inositol(1,4,5)triphosphate.

3. Assay for evaluating the lipid affinity and selectivity of biological and synthetic receptors.
A new fluorescent indicator displacement assay (IDA) will be developed to facilitate fast and accurate evaluation of the affinity of unlabeled receptors for lipid-bound inositol phosphates while consuming fewer resources than techniques such as ITC. The assay is expected to be useful for studying the lipid selectivity of both synthetic and biological (protein) receptors for various phosphatidylinositides. This project uses instrumentation for fluorescence measurements in both cuvettes and microplates.

Group Members

Dr. Kasey J. Clear

Dr. Clear is a native of Niles, Michigan and was awarded his B.S. in Chemistry from Indiana University South Bend in 2011. He completed his Ph.D. in 2016 at the University of Notre Dame under the supervision of Professor Bradley D. Smith, where he studied anion and biomembrane molecular recognition using synthetic receptors and fluorescent dyes. As a graduate student, Dr. Clear co-authored six peer-reviewed articles and one book chapter. He began his independent career in academia at Murray State University in fall of 2016. His research interests include the design and study of synthetic receptors for biologically important anions that will have application in separations, imaging, and chemical biology. Dr. Clear primarily teaches organic chemistry at both the undergraduate and graduate level. He is currently teaching or has taught the following courses at MSU:
CHE 105 - Introductory Chemistry (team taught)
CHE 210 - Brief Organic Chemistry
CHE 215 - Brief Organic Chemistry Laboratory
CHE 312 - Organic Chemistry I with Laboratory
CHE 320 - Organic Chemisty II
CHE 325 - Organic Chemistry II Laboratory
CHE 517/617 - Advanced Organic Chemistry
In addition, Dr. Clear is the seminar coordinator for the chemistry department (CHE 601/602).
To contact:
Department of Chemistry
Murray State University
1232 Jesse D. Jones Hall (office, lab is room 1233)
Office phone: (270) 809-6597 (office phone)
Email: kclear@murraystate.edu


Josh Batson

Major: Chemistry
Future Plans:

Random info about Josh:


Erin Calvert

Major: Chemistry Pre-Med
Future Plans:

Random info about Erin:


Natalie Jarrett

Major: Chemistry


Bailey Morales

Major: Chemistry


Gena Wilson

Major: Chemistry
Future plans:

Random info about Gena:

 

Past Members:
Hannah Adamson (BSA Vet Tech); Current position: DVM program at Ross University School of Veterinary Medicine
Shelby Wakefield (BS Chemistry); Current position: Chemistry Ph.D program at University of Wyoming
Dmitriy Bachynsky, graduate student
Jacob Meadows, B.S. Chemistry

Prospective Students:
Motivated undergraduate and graduate students who are interested in doing research are welcome to join. If you are interested, contact Dr. Clear by e-mail (kclear@murraystate.edu) or just stop by his office (1232 CB).

Publications

Graduate Publications:
7. Harmatys, K. M., Musso, A. J., Clear, K. J., Smith, B. D. (2016) Small molecule additive enhances cell uptake of 5-aminolevulinic acid and conversion to protoporphyrin IX. Photochem. Photobiol. Sci. Advance Article, DOI: 10.1039/C6PP00151C Link
6. Rice, D. R., Clear, K. J., Smith, B. D. (2016) Imaging and therapeutic applications of zinc(ii)-dipicolylamine molecular probes for anionic biomembranes. Chem. Commun. 52, 8787-8801 Link
5. Clear, K. J., Virga, K., Gray, L., and Smith, B. D. (2016) Using membrane composition to fine-tune the pKa of an optical liposome pH sensor. J. Mater. Chem. C 4, 2925-2930 Link
4. Clear, K. J., Harmatys, K. M., Rice, D. R., Wolter, W. R., Suckow, M. A., Wang, Y., Rusckowski, M., and Smith, B. D. (2016) Phenoxide-bridged zinc(II)-bis(dipicolylamine) probes for molecular imaging of cell death. Bioconjugate Chem. 27, 363-375 Link
3. Clear, K. J. and Smith, B. D. (2015) Synthetic Receptors for Polar Lipids. Synthetic Receptors for Biomolecules, Monographs in Supramolecular Chemistry, Royal Society of Chemistry: pp 405-436 Link
2. Plaunt, A. J., Clear, K. J., and Smith, B. D. (2014) 19F NMR indicator displacement assay using a synthetic receptor with appended paramagnetic relaxation agent. Chem. Commun. 50, 10499-10501 Link
1. Clear, K. J., Stroud, S., and Smith, B. D. (2013) Dual colorimetric and luminescent assay for dipicolinate, a biomarker of bacterial spores. Analyst 138, 7079-7082 Link

Group Photos

April 2018: Chemistry Night at Murray Elementary School (featuring Gena Wilson and other chem students)
Murray Elementart School visit

March 2018: Dr. Clear with Hannah Adamson and Shelby Wakefield by their poster at the Spring 2018 ACS National Meeting